Conjugation
Rp4 Phyto

Part:BBa_K2657002:Design

Designed by: Ryan Bailey   Group: iGEM18_Austin_UTexas   (2018-10-09)


Rp4 Origin of Transfer PhytoBrick


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4


Design Notes

Very specific primers were necessary to ensure proper conversion between Golden Gate and MoClo standards.


Source

BBa_K2657002 was isolated from Golden Gate compatible rp4 oriT part plasmid from the Barrick Lab.

References